The College of Education for Pure Sciences at the University of Basrah  discussed a master's thesis on life sciences on (research
On the role of essential oil in pi3k/akt/mtor signaling pathways of cancer cell lines
and natural)
The thesis presented by the researcher (Zainab Hussein Ali) included
Current conventional therapies such as surgery, radiotherapy, or chemotherapy are used to prolong it
The patient's age often fails due to the body's resistance to drugs and poor tissue selectivity rose
Because of the side effects, so the need to develop new treatment strategies in order to reduce the effects
Side effect and improve the selectivity of treatment and reduce the spread of the tumor and control it. As the inhibition of cells without
Killing it is one of the new strategies that researchers are seeking to apply
from its spread.
It targets the pi3k/akt/mtor angiogenesis pathway, which is a regulator of the physiological processes of cells as one
Novel strategies to prevent tumor proliferation and progression. Some of the compounds that were obtained are shown
It has promising results from natural sources in the treatment of cancer, including betel nut essential oil
Its leaves are the vital parts of the plant, which have tremendous therapeutic values, as the oil has medicinal properties
As anti-bacterial, anti-fungal, anti-inflammatory and antioxidant.
conclusions
and the PI3K/AKT/MTOR signaling pathway was not affected upon treatment with metformin in SK-cells
GT-4 at the level of gene expression, in contrast to the presence of cytotoxicity of metformin at concentration IC50
25mM, which indicates that metformin inhibits esophageal cancer cells by a distant pathway
on the PI3K/AKT/MTOR cell angiogenesis pathway.
In the inhibition of both PI3K and MTOR and confirmed by their inhibition in co-treatment

And oil has a role
combination when compared with metformin treatment, which did not affect stimulation or inhibition
The two genes above, as well as the results obtained from the Composyn isobologram program
gave evidence of synergistic action in the last three concentrations.
And that the gene expression of AKT gene increased in esophageal cancer cells when treated with oil alone and
In combination with metformin, this indicates that it works in other signaling pathways that appear to be activated
This gene does not affect MTOR inhibition. Syringes in the normal line occurred at four concentrations, the percentage of inhibition at three combined concentrations
More than 70%, then this indicates a significant effect on the normal cell line when the oil is involved
With metformin as a treatment strategy. It was observed when the oil was involved with Metformin lies an effect
synergistic
Significant synergy on colon cancer cell line, as it was noticed that four concentrations gave effective effect
And the rates of inhibition ranged from 80 - 58%, which indicates a high effectiveness involving the two substances together.
And that metformin affects the inhibition of PI3K/MTOR cell reproduction when treated with oil
Thus, it can be given to patients with type 2 diabetes who have esophageal cancer
Metformin with cytogenetic pathway inhibitors.
And if we take into account the results of the examination using the AO/EB stain and gene expression, and so on
There must be another pathway that led to the inhibition of metformin-treated cells, which means that metformin
It does not necessarily work in different cancers by the same mechanism to inhibit it.
It is clear from the results of the current study that stimulation of the programmed cell death pathway contradicts inhibition
for him.
cytogenetic pathway and may be complementary
And that the oil is stronger in its effect on the cancerous cell line than metformin because it stimulates the cancer pathway
Programmed cell death, on the one hand, and inhibits the path of cell reproduction, on the other hand, that is, the oil has
Anti-cancer activity more than metformin because it inhibits tumor by two different pathways.
Recommendations
It is recommended to try betel oil in cancerous tumors.in vivio
And an experiment with betel oil on the RAS/RAF/MEK/ERK reproductive pathway. Studying the mechanism of the oil's effect on cancer cell lines using molecular techniques.
And study the effect of metformin on the cell life cycle and the reproductive pathway RAS/RAF/MEK/ERK on
SK-GT-4. cellular line
It is recommended that metformin be tested on other cancer cell lines and its role in the oncogenesis pathway
PI3k/AKT/MTOR.
It is recommended to investigate the relationship of oil and PTEN gene regulating the feedback pathway of cell reproduction.
And there is no effect of betel oil on cervical cancer HeLa, so we recommend the study not to
Its use in studies related to the pharmaceutical industry against cancer