The College of Education for Pure Sciences at the University of Basrah  discussed a master’s thesis on a molecular diagnostic study of algae in saline ponds Dunaliella sp. in Basrah  Governorate.
The thesis presented by the researcher (Anfal Falah Abdullah), due to the lack of studies related to the phenotypic and molecular diagnostic study of the salt-tolerant green alga Dunaliella sp. In the province of Basra in particular and Iraq in general, and because of its wide spread in the province, especially in the salty ponds, the current study decided to shed light on it.
message aim
 Isolation, purification and culture of Dunaliella sp. From some of the brine ponds in the province of Basra.
 Measuring some important environmental factors such as water and air temperature, salinity and pH, and showing their relationship and impact on the phenotypic shape of algae.
and diagnosis of the moss Dunaliella sp. Apparently for each water sample from different ponds in the governorate to show the similarity and difference between algal isolates in their ability to produce carotene and grow in vitro.
And the identification of isolated species of algae at the molecular level using the technique of polymerase chain reaction (PCR) and depending on the genes specific to this genus and type D. salina in particular.
And conducting the analysis of DNA sequences to establish sex, gender or isolation in the different study areas and recording it in the Gene bank and drawing the evolutionary tree.
The message was deduced
It was concluded from the current study that all the strains in the nine sites in Basra Governorate, which inhabit
 The saline ponds, in terms of phenotypic classification, all belong to the species D. salina.
The physiological study of algae showed that all strains had similar growth under salinity concentration of 5-15% of sodium chloride salt, and they also had the same mechanism in producing carotene with an increase in salinity concentration in addition to the possibility of producing carotene in environments free from salinity and this is evidence that all of these strains
It belongs to one species, and its strains are numerous.
The study showed that the primers (MA2-DSc) have a very high efficiency in molecularly diagnosing the alga D. salina in all its strains, and that the current study is the first of its kind in Iraq in confirming this genus and species as D. salina, adopting the highest phenotypic, physiological and molecular characteristics.
And the study proved, through the results of genetic diagnosis, and based on the analysis of the sequence of nitrogenous bases of the products of the polymerase chain reaction and the diagnostic gene 18s rRNA and drawing the genetic kinship tree, definitively and definitively that the alga under study and for all the strains studied is the type D. salina and its different strains recorded in the current study and it is completely isolated from Green algae Chlamydomonas and all kinds
Recommended message
We recommend conducting a physiological and molecular study by culturing the alga in the laboratory and showing the effect of salinity
 On stimulating salt-resistant genes, such as the genes responsible for the production of glycerol and carotene, and the possibility of using them economically.
The study recommends conducting a phenotypic, physiological and molecular diagnosis of algae in the rest of Iraq's governorates to confirm this in the local and global database.
The study also recommends paying attention to isolating and diagnosing marine species morphologically and molecularly belonging to the members of this genus in the waters of the Arabian Gulf and the Shatt al-Arab estuary because it did not shed light on them at all.