A doctoral thesis at the University of Basrah discussing about (preparation and diagnosis of some of the derivatives of 2_ thioxoamidazolidines_4_on and the study of its biological activity)

The thesis was discussed in the College of Education for Pure Sciences, Department of Chemistry, University of Basra, on the preparation and characterization of some of the derivatives of 2-Thioxoamidazolidine-4-ON and the study of its biological activity.
The thesis presented by the researcher (Laila Adnan Abdel-Jabbar) dealt with the preparation of heterocyclic compounds known as 2-thiooxamidazolidine-4-un by reacting maleamide derivatives with the substituted isothiocyanate either with hexyl ring or phenyl ring, the activity was studied against three types of bacteria and two types of fungi. The effect of the compounds as an anti-oxidant was studied, and ascorbic acid was used as a positive control agent. The toxicity of the compounds against breast and liver cancer cells was studied by comparing the half-inhibitory concentration of the compounds with the half-inhibited concentration of cisplatin, which is considered a positive control agent against breast and liver cancer cells. Then the effect of the selected compounds was studied. cancerous cells programmed death,
The thesis was concluded
All the prepared compounds did not show activity against E.coli bacteria except compound 5b. Compound 5h did not show any activity against all studied microorganisms. Compounds 5a, 5b, 5c, and 5e showed good activity against C. albicans and compounds 5a-g. Good towards A.niger mushroom.
Compounds 5a, 5b, 5c, 5h and compounds 7e, 7f, 7g, 7h did not show any anti-oxidative activity, while the compounds 5d, 5e, 5f, 5g showed weak anti-oxidative activity.
The preliminary examination of the prepared cyclic compounds showed that compounds 5a, 5b, 5d-h had anti-toxic activity against human cancer cells, and compounds 5a-h, 7f had toxic activity against human hepatocytes.
The compound 5e showed a more toxic effect on the shape of the liver cancer cells than the toxic effect shown by the compounds 5d and 5h on the shape of the cancerous liver cells, and the compound 5e also showed a more toxic effect than the compound 5h on the breast cancer cells and based on the values ​​of the half-inhibitory concentrations of the studied compounds we conclude that The compound that has the lowest value of the half-inhibited concentration has the most toxic effect on the shape of the cancer cells (the toxicity effect of the studied compounds was compared with that of cancer cells not treated with Contorol)).
The results of the study of the semi-inhibitory concentration of the two compounds 5e, 5h on stopping the cell cycle of human breast cancer cells by flow cytometry method showed the effectiveness of the two compounds 5e, 5h on stopping the life cycle of breast cancer cells, as it was observed that the two compounds stop the life cycle of breast cancer cells at the G2 phase At the G2/M checkpoint.
6- The results of a study of the semi-inhibitory concentration of the compounds 5e, 5d, 5h on stopping the cell cycle of human liver cancer cells by flow cytometry method showed their effectiveness in stopping the cycle of hepatoma cells. Checkpoint G1/S)).
The results showed the effect of the half-inhibitory concentration of the two compounds 5e,5h on the programmed cell death of human breast cancer cells. These compounds cause the programmed cell death of breast cancer cells.
The results showed the effect of the half-inhibited concentration of compounds 5e, 5d, 5h on programmed cell death of human liver cancer cells by flow cytometry method. These compounds cause apoptosis of liver cancer cells.
recommended thesis
Preparation of compounds derived from the prepared cyclic compounds such as the preparation of Thio Sulphonic ester or Mannich Yield product.
Expanding the study of the effectiveness of biologically prepared compounds on other types of pathogenic bacteria and fungi.
And studying the effect of compounds that have anti-carcinogenic activity on mice carrying breast and liver cancer cells (in vevo).
Expanding the study of the carcinogenic activity of the prepared cyclic compounds for other types of cancer cells.